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KMID : 1149220130210020133
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Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 2 p.133 ~ p.143
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Simultaneous Analysis of Bangpungtongseong-san and Its Antioxidant Effect
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Seo Chang-Seob
Kim Ohn-Soon
Shin Hyeun-Kyoo
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Abstract
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Objectives : We carry out the simultaneous quantification for quality control of four components in Bangpungtongseong-san
(BPTSS) sample. In addition, we assessed the antioxidant effects of BPTSS sample.
Methods : The used column for separation and analysis of four compounds was Luna C18 column and column oven temperature was maintained at 40℃. The mobile phase for simultaneous determination consisted of two solvent systems, 1.0% acetic acid in water and 1.0% acetic acid in acetonitrile. High performance liquid chromatography-photodiode array (HPLC-PDA) method for analysis was performed at a flow rate of 1.0 mL/min with PDA detection at 254 and 280 nm. The injection volume was 10 μL. The antioxidant activities of BPTSS were evaluated by measuring free radical scavenging activities on 2,2'-Azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The inhibitory effects on low-density lipoprotein (LDL) oxidation were evaluated by the formation of thiobarbituric acid relative substances (TBARS) and relative electrophoretic mobility (REM).
Results : Calibration curves were acquired with r2 ≥ 0.9999. The values of limit of detection (LOD) and quantification (LOQ) were 0.06?0.29 μg/mL and 0.20?0.98 μg/mL, respectively. The amounts of geniposide, liquiritin, baicalin, and glycyrrhizin in BPTSS were 5.06, 7.33, 27.56, and 7.81 mg/g, respectively. The BPTSS showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction (RC50) against ABTS and DPPH radicals were 72.51 μg/mL and 128.49 μg/mL. Furthermore, GMGHT reduced the oxidation properties of LDL induced by CuSO4.
Conclusions : The established HPLC-PDA method will be helpful to improve quality control of BPTSS. In addition, BPTSS has potentials as therapeutic agent on anti-atherosclerosis.
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KEYWORD
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Bangpungtongseong-san, Simultaneous Analysis, Antioxidant Effect, HPLC-PDA, Low-Density Lipoprotein
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